Ghandour M S, Derer P, Labourdette G, Delaunoy J P, Langley O K
J Neurochem. 1981 Jan;36(1):195-200. doi: 10.1111/j.1471-4159.1981.tb02395.x.
The glial cell contents of S100 protein, 2',3'-cyclic AMP, 3'-phosphohydrolase (CNP), isoenzyme II of carbonic anhydrase (CAII) and butyrylcholinesterase (BuChE) were biochemically determined in the cerebellum and cerebrum of the reeler mutant mouse. Astrocytes and oligodendrocytes, shown by this study, contain abnormal amounts of these components. The CAII concentration was significantly increased in the particulate fraction of the reeler cerebellum and cerebrum (by 50% and 89%, respectively). The BuChE specific activity was greatly increased in the reeler, by 120% for cerebellum and by 40% in cerebrum. In contrast, the S100 protein concentration was reduced in the reeler cerebellum by 40% and by 25% in cerebrum, while the CNP specific activity increased by 30% in the reeler cerebellum. In addition, the glial cell distribution was studied by immunohistological techniques with antibodies directed against S100 protein, glial fibrillary acidic protein (GFA) and CAII. Apparently the density of glial cells is not significantly affected. However, the Golgi epithelial cells were usually abnormally placed and their Bergmann fibres were less well developed.
对reeler突变小鼠的小脑和大脑进行了生化测定，以检测其中S100蛋白、2',3'-环磷酸腺苷、3'-磷酸水解酶（CNP）、碳酸酐酶同工酶II（CAII）和丁酰胆碱酯酶（BuChE）的胶质细胞含量。本研究表明，星形胶质细胞和少突胶质细胞中这些成分的含量异常。在reeler小鼠的小脑和大脑颗粒部分，CAII浓度显著增加（分别增加50%和89%）。reeler小鼠中BuChE的比活性大幅增加，小脑增加120%，大脑增加40%。相比之下，reeler小鼠小脑中S100蛋白浓度降低40%，大脑中降低25%，而reeler小鼠小脑中CNP的比活性增加30%。此外，采用针对S100蛋白、胶质纤维酸性蛋白（GFA）和CAII的抗体，通过免疫组织学技术研究了胶质细胞的分布。显然，胶质细胞的密度没有受到显著影响。然而，高尔基上皮细胞的位置通常异常，其伯格曼纤维发育较差。
