Hamana K, Zama M
Nucleic Acids Res. 1980 Nov 25;8(22):5275-88. doi: 10.1093/nar/8.22.5275.
The possible role of LG-1, a Tetrahymena specific HMG protein found in the macronuclear chromatin (Hamana, K. and Iwai, K. (1979) J. Biochem. 86, 789-794), was examined in relation to the chromatin structure. The chromatin isolated from cells synchronized at different stages of the cell cycle contained about one molecule of LG-1 per nucleosome. Limited digestion of the chromatin with DNase I or micrococcal nuclease selectively released LG-1 with the nucleosomal core histones and H1 remained insoluble, bound to the resistant DNA. Depending on the cell stages several types of chromatin structure were distinguished by their nuclease sensitivity. However, the chromatin at different stages exhibited the similar behavior of the LG-1 release with the nucleases as a function of the degree of chromatin solubilization. The results suggest that LG-1 proteins play a role in the chromatin organization which is rather independent of the cell stages.
在大核染色质中发现的一种四膜虫特异性HMG蛋白LG-1（滨名和岩井，1979年，《生物化学杂志》86卷，789 - 794页），其与染色质结构相关的潜在作用已被研究。从细胞周期不同阶段同步化的细胞中分离出的染色质，每个核小体约含一个LG-1分子。用DNase I或微球菌核酸酶对染色质进行有限消化，可选择性地释放出LG-1，而核小体核心组蛋白和H1仍不溶，与抗性DNA结合。根据细胞阶段，几种染色质结构类型因其对核酸酶的敏感性而得以区分。然而，不同阶段的染色质在LG-1随核酸酶释放方面，表现出与染色质溶解程度相关的相似行为。结果表明，LG-1蛋白在染色质组织中发挥作用，且该作用相当独立于细胞阶段。
