转座DNA元件IS2内的缺失和DNA重排。通过DNA修复合成产生回文DNA的模型。
Deletions and DNA rearrangements within the transposable DNA element IS2. A model for the creation of palindromic DNA by DNA repair synthesis.
作者信息
Besemer J, Görtz G, Charlier D
出版信息
Nucleic Acids Res. 1980 Dec 11;8(23):5825-33. doi: 10.1093/nar/8.23.5825.
Abstract
Three derivatives of mutant ga10P-308::IS2-I of Escherichia coli were characterized by DNA sequence analysis. Deletions and DNA sequence rearrangements were observed which apparently were initiated at short A-T rich inverted repeats within IS2. Two of the mutants carried newly synthesized DNA sequences which were inverted copies of already existing IS2 sequences. Thus long stretches with twofold symmetry were formed. It is discussed whether these inverted repeats were formed by DNA repair synthesis which was initiated at the A-T rich palindromes of IS2.
摘要
通过DNA序列分析对大肠杆菌突变体ga10P - 308::IS2 - I的三种衍生物进行了表征。观察到缺失和DNA序列重排，这些重排显然起始于IS2内富含A - T的短反向重复序列。其中两个突变体携带新合成的DNA序列，这些序列是现有IS2序列的反向拷贝。因此形成了具有双重对称性的长片段。本文讨论了这些反向重复序列是否由在IS2富含A - T的回文序列处起始的DNA修复合成形成。
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