Terent A, Ronquist G
Acta Neurol Scand. 1980 Dec;62(6):327-35. doi: 10.1111/j.1600-0404.1980.tb03045.x.
Adenylate kinase activity was found in 32 of 34 samples of cerebrospinal fluid (CSF) from 21 patients with stroke and seven patients with global cerebral ischaemia (GCI). The light absorbance values of the spectrum 400-650 nm revealed the scanty occurrence of haemoglobin products in the CSF in some patients. No correlation was found between the absorbance values at 415 nm, reflecting oxyhaemoglobin, and the adenylate kinase activities. Thus, a main contribution to the adenylate kinase activity in CSF by leakage of this enzyme from erythrocytes could be ruled out. Instead increased leakiness of the brain cells, having an impaired metabolism due to insufficient supply of oxygen and glucose, was the most plausible cause of the findings. The quotient between the adenylate kinase activity and the light absorbance at 415 nm seemed to reflect the extent of ischaemically deranged brain tissue in the GCI patients, while the CSF-lactate values were not correlated to the clinical outcome. Glutathione, an intracellular tripeptide, was more often found in the CSF from GCI patients than from stroke patients.
在21例中风患者和7例全脑缺血（GCI）患者的34份脑脊液（CSF）样本中，有32份检测到腺苷酸激酶活性。400 - 650 nm光谱的吸光度值显示，部分患者脑脊液中血红蛋白产物含量稀少。在反映氧合血红蛋白的415 nm处的吸光度值与腺苷酸激酶活性之间未发现相关性。因此，可以排除红细胞中该酶泄漏对脑脊液中腺苷酸激酶活性的主要贡献。相反，由于氧气和葡萄糖供应不足导致代谢受损的脑细胞通透性增加，是这些发现最合理的原因。腺苷酸激酶活性与415 nm处的吸光度之比似乎反映了GCI患者缺血性紊乱脑组织的程度，而脑脊液乳酸值与临床结果无关。谷胱甘肽是一种细胞内三肽，在GCI患者的脑脊液中比中风患者更常见。
