用亲和纯化的鸟嘌呤核苷酸调节蛋白使鸟嘌呤核苷酸和氟化物刺激的活性恢复到腺苷酸环化酶缺陷细胞系中。
Restoration of guanine nucleotide- and fluoride-stimulated activity to an adenylate cyclase-deficient cell line with affinity-purified guanine nucleotide regulatory protein.
作者信息
Nielsen T B, Downs R W, Spiegel A M
出版信息
Biochem J. 1980 Aug 15;190(2):439-43. doi: 10.1042/bj1900439.
Abstract
A guanine nucleotide-binding protein purified from turkey erythrocytes by affinity chromatography confers both F-- and guanine nucleotide-stimulation of adenylate cyclase to membranes from CYC- cells, a mutant cell line deficient in these responses. Interaction of turkey erythrocyte membranes with beta-adrenergic agonists before affinity chromatography, which is essential for binding of the guanine nucleotide regulatory protein to the affinity matrix, was also required for recovery of F--stimulation restoring activity in the affinity eluate.
摘要
通过亲和层析从火鸡红细胞中纯化得到的一种鸟嘌呤核苷酸结合蛋白，能使CYC-细胞（一种缺乏这些反应的突变细胞系）的膜产生F-和鸟嘌呤核苷酸对腺苷酸环化酶的刺激作用。在亲和层析之前，火鸡红细胞膜与β-肾上腺素能激动剂的相互作用对于鸟嘌呤核苷酸调节蛋白与亲和基质的结合至关重要，同时对于亲和洗脱液中恢复F-刺激恢复活性也是必需的。
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2
The role of the guanine nucleotide exchange reaction in the regulation of the beta-adrenergic receptor and in the actions of catecholamines and cholera toxin on adenylate cyclase in turkey erythrocyte membranes.鸟嘌呤核苷酸交换反应在β-肾上腺素能受体调节以及儿茶酚胺和霍乱毒素对火鸡红细胞膜腺苷酸环化酶作用中的角色。
J Biol Chem. 1980 Feb 10;255(3):988-95.
3
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