Sternweis P C, Gilman A G
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4888-91. doi: 10.1073/pnas.79.16.4888.
Activation of the purified guanine nucleotide-binding regulatory component (G/F) of adenylate cyclase by F- requires the presence of Mg2+ and another factor. This factor, which contaminates commercial preparations of various nucleotides and disposable glass test tubes, has been identified as Al3+. In the presence of 10 mM Mg2+ and 5 mM F-, AlCl3 causes activation of G/F with an apparent activation constant of approximately 1-5 muM. The requirement for Al3+ is highly specific; of 28 other metals tested, only Be2+ promoted activation of G/F by F-.
F-激活腺苷酸环化酶的纯化鸟嘌呤核苷酸结合调节成分(G/F)需要Mg2+和另一种因子的存在。这种因子存在于各种核苷酸的商业制剂和一次性玻璃试管中,已被鉴定为Al3+。在10 mM Mg2+和5 mM F-存在的情况下,AlCl3可激活G/F,其表观激活常数约为1-5 μM。对Al3+的需求具有高度特异性;在测试的其他28种金属中,只有Be2+能促进F-对G/F的激活。