铝：氟化物激活腺苷酸环化酶调节成分所必需的物质。
Aluminum: a requirement for activation of the regulatory component of adenylate cyclase by fluoride.
作者信息
Sternweis P C, Gilman A G
出版信息
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4888-91. doi: 10.1073/pnas.79.16.4888.
Abstract
Activation of the purified guanine nucleotide-binding regulatory component (G/F) of adenylate cyclase by F- requires the presence of Mg2+ and another factor. This factor, which contaminates commercial preparations of various nucleotides and disposable glass test tubes, has been identified as Al3+. In the presence of 10 mM Mg2+ and 5 mM F-, AlCl3 causes activation of G/F with an apparent activation constant of approximately 1-5 muM. The requirement for Al3+ is highly specific; of 28 other metals tested, only Be2+ promoted activation of G/F by F-.
摘要
F-激活腺苷酸环化酶的纯化鸟嘌呤核苷酸结合调节成分（G/F）需要Mg2+和另一种因子的存在。这种因子存在于各种核苷酸的商业制剂和一次性玻璃试管中，已被鉴定为Al3+。在10 mM Mg2+和5 mM F-存在的情况下，AlCl3可激活G/F，其表观激活常数约为1-5 μM。对Al3+的需求具有高度特异性；在测试的其他28种金属中，只有Be2+能促进F-对G/F的激活。
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Purification of the regulatory component of adenylate cyclase.腺苷酸环化酶调节成分的纯化
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The regulatory component of adenylate cyclase. Purification and properties.腺苷酸环化酶的调节成分。纯化及特性
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