Nicholls P, Chanady G A
Biochim Biophys Acta. 1981 Feb 12;634(2):256-65. doi: 10.1016/0005-2728(81)90144-4.
The 607 nm complex of cytochrome c oxidase, formed aerobically in the presence of CO, appears as an intermediate during the oxidation of CO to CO2 by the enzyme. Maximal steady-state formation of this complex requires oxygen, high levels of carbon monoxide, and the presence of an endogenous hydrogen donor system or the addition of small amounts of reductant (both with isolated enzyme and mitochondrial preparations). The 607 nm complex can be formed after removing CO from the mixed-valence CO complex (cytochrome a3+a2(3)+CO) by aerating the presumably CO-free product. The elements of CO are, therefore, probably not part of the 607 nm complex nor of the related "compound C" produced at low temperatures.
细胞色素c氧化酶的607纳米复合物在有氧条件下于一氧化碳存在时形成，在该酶将一氧化碳氧化为二氧化碳的过程中表现为一种中间体。该复合物的最大稳态形成需要氧气、高浓度一氧化碳以及内源性氢供体系统的存在或添加少量还原剂（在分离的酶和线粒体制剂中均如此）。通过对推测不含一氧化碳的产物进行曝气，从混合价态一氧化碳复合物（细胞色素a3 + a2(3)+CO）中去除一氧化碳后，可形成607纳米复合物。因此，一氧化碳的成分可能既不是607纳米复合物的一部分，也不是在低温下产生的相关“化合物C”的一部分。
