Morgan J E, Blair D F, Chan S I
J Inorg Biochem. 1985 Mar-Apr;23(3-4):295-302. doi: 10.1016/0162-0134(85)85038-8.
When pulsed cytochrome c oxidase is exposed to carbon monoxide in the absence of oxygen the enzyme is converted quickly to its CO-associated mixed valence state. The half-time for this reaction at 0 degree C is about 4 min. This is about 100 times faster than a similar reaction which begins with the resting form of the enzyme. The possible significance of this reaction in understanding the pulsed/resting phenomenon and the carbon monoxide oxygenase reactions of cytochrome oxidase is discussed.
当脉冲细胞色素c氧化酶在无氧条件下暴露于一氧化碳时，该酶会迅速转化为其与一氧化碳结合的混合价态。此反应在0℃时的半衰期约为4分钟。这比从酶的静止形式开始的类似反应快约100倍。讨论了该反应在理解脉冲/静止现象以及细胞色素氧化酶的一氧化碳加氧酶反应中的可能意义。
