Edlind T D, Ihler G M
J Bacteriol. 1981 Mar;145(3):1436-41. doi: 10.1128/jb.145.3.1436-1441.1981.
Single strands of EcoRI-cleaved pCR1 deoxyribonucleic acid were examined by electron microscopy for intrastrand base pairing by using partial denaturing conditions. The locations of three stem and loop structures were mapped relative to the inverted repeat of Tn903. Potential roles and origins of these loops are discussed.
通过电子显微镜,在部分变性条件下,对经EcoRI切割的pCR1脱氧核糖核酸单链进行链内碱基配对检测。相对于Tn903的反向重复序列,绘制了三个茎环结构的位置。讨论了这些环的潜在作用和起源。