Perry M, Chalkley R
J Biol Chem. 1981 Apr 10;256(7):3313-8.
We have examined the effects of histone hyperacetylation upon nuclease digestion of nuclei and subsequent fractionation of chromosomal material in the presence of MgCl2. DNase I shows a maximum sensitivity towards hyperacetylated nuclei at somewhat elevated ionic strengths (150-200 mM NaCl), whereas micrococcal nuclease exhibits no specificity for acetylated nuclei over a broad range of ionic strengths. Fractionation in the presence of MgCl2 of hyperacetylated nuclei digested with micrococcal nuclease results in a substantial increase in the amount of soluble chromatin relative to that obtained with control nuclei. This increased yield of Mg2+-soluble chromatin results from the recruitment into this fraction of oligonucleosomes containing extremely hyperacetylated histones. These results suggest that contiguous nucleosomes containing highly acetylated histones may be altered in their ability to interact with themselves and with other nucleosomes.
我们研究了组蛋白高度乙酰化对细胞核核酸酶消化以及随后在MgCl2存在下对染色体物质进行分级分离的影响。在离子强度略有升高（150 - 200 mM NaCl）时，DNase I对高度乙酰化的细胞核表现出最大敏感性，而微球菌核酸酶在广泛的离子强度范围内对乙酰化的细胞核没有特异性。用微球菌核酸酶消化的高度乙酰化细胞核在MgCl2存在下进行分级分离，相对于对照细胞核，可溶性染色质的量大幅增加。Mg2 + - 可溶性染色质产量的增加是由于含有极度高度乙酰化组蛋白的寡核小体被招募到该级分中。这些结果表明，含有高度乙酰化组蛋白的相邻核小体在与自身及其他核小体相互作用的能力上可能会发生改变。
