Yang M S, Passonneau P, Lowry O H
J Neurochem. 1981 Mar;36(3):1272-8. doi: 10.1111/j.1471-4159.1981.tb01727.x.
Seventy-one regions of mouse brain, and many subdivisions of some of these, were analyzed for cyclic nucleotide phosphodiesterase. The samples were dissected from lyophilized frozen sections. Since the average sample weighed only 25 ng (20 X 75 X 75 mu3), regions as small as the locus ceruleus could be analyzed. Activities in gray areas ranged 40-fold from a high in the pars reticulata of the substantia nigra to a low in the deep cerebellar nuclei. The activity in fiber tracts also varied about 40-fold, and on a lipid-free dry weight basis was similar to the activity in the gray matter where the fibers originated. The rank order for gray regions was basal ganglia, amygdala, hippocampus, cerebral cortex, most of the diencephalic nuclei, nuclei of the pons, cerebellum, and nuclei of the medulla.
对小鼠大脑的71个区域以及其中一些区域的许多亚区进行了环核苷酸磷酸二酯酶分析。样本取自冻干的冰冻切片。由于平均样本重量仅为25纳克（20×75×75立方微米），像蓝斑这样小的区域也能进行分析。灰质区域的活性范围为40倍，从黑质网状部的高活性到小脑深部核团的低活性。纤维束中的活性也有大约40倍的变化，以无脂干重为基础，其活性与纤维起源的灰质中的活性相似。灰质区域的活性排序为基底神经节、杏仁核、海马体、大脑皮层、大多数间脑核、脑桥核、小脑和延髓核。
