Nimit Y, Skolnick P, Daly J W
J Neurochem. 1981 Mar;36(3):908-12. doi: 10.1111/j.1471-4159.1981.tb01680.x.
The endogenous levels of adenosine functionally linked to cyclic AMP systems in rat cerebral cortical slices are regulated by both adenosine deaminase and adenosine uptake systems. 2'-Deoxycoformycin (2'-DCF), an adenosine deaminase inhibitor, slightly increased basal, adenosine, and norepinephrine-elicited accumulations of cyclic AMP, whereas dipyridamole, an uptake inhibitor, had an even greater effect on cyclic AMP accumulations under the same conditions. Combinations of 2'-DCF and dipyridamole elicited a greater effect than either compound alone. Neither 2'-DCF nor dipyridamole significantly augmented accumulations of cyclic AP elicited by a depolarizing agent, veratridine, suggesting that the adenosine "released" during neuronal depolarization of brain slices is not as subject to inactivation by uptake or deamination as endogenous adenosine in control brain slices. The accumulation of cyclic AMP elicited by a combination of norepinephrine and veratridine was greater than additive. The response to a pure beta-adrenergic agonist, isoproterenol, was not potentiated by 2'-DCF, dipyridamole, or veratridine, consonant with minimal interaction of endogenous adenosine with beta-adrenergic systems.
与大鼠大脑皮层切片中环磷酸腺苷（cAMP）系统功能相关的内源性腺苷水平受腺苷脱氨酶和腺苷摄取系统的调节。腺苷脱氨酶抑制剂2'-脱氧助间型霉素（2'-DCF）略微增加了基础状态下、腺苷以及去甲肾上腺素引发的cAMP积累，而摄取抑制剂双嘧达莫在相同条件下对cAMP积累的影响更大。2'-DCF和双嘧达莫联合使用产生的效果比单独使用任一化合物都要大。2'-DCF和双嘧达莫均未显著增强由去极化剂藜芦定引发的环磷腺苷（cyclic AP）积累，这表明在脑片神经元去极化过程中“释放”的腺苷不像对照脑片中的内源性腺苷那样容易受到摄取或脱氨作用的失活影响。去甲肾上腺素和藜芦定联合引发的cAMP积累大于两者相加的效果。对纯β-肾上腺素能激动剂异丙肾上腺素的反应未被2'-DCF、双嘧达莫或藜芦定增强，这与内源性腺苷与β-肾上腺素能系统的相互作用最小一致。
