环磷酸腺苷受体蛋白与乳糖操纵基因DNA相互作用。
Cyclic AMP receptor proteins interacts with lactose operator DNA.
作者信息
Schmitz A
出版信息
Nucleic Acids Res. 1981 Jan 24;9(2):277-92. doi: 10.1093/nar/9.2.277.
Abstract
The wildtype lac promoter-operator region of E. coli was reinvestigated for cyclic AMP receptor protein (CRP) binding sites using the 'footprinting' method. Two distinct interaction sites were found, one in the -60 base pair region, which has previously been described, and a second one covering the lac operator. These two sites are similar in extent, orientation and the characteristic pattern of CRP-induced DNaseI enhanced cutting sites.
摘要
利用“足迹法”对大肠杆菌野生型乳糖启动子-操纵子区域进行了重新研究，以寻找环磷酸腺苷受体蛋白（CRP）的结合位点。发现了两个不同的相互作用位点，一个位于-60碱基对区域，该区域此前已有描述，另一个覆盖乳糖操纵子。这两个位点在范围、方向以及CRP诱导的DNaseI增强切割位点的特征模式方面相似。
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