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环磷酸腺苷受体蛋白与乳糖操纵子启动子的相互作用。

Interaction of the cAMP receptor protein with the lac promoter.

作者信息

Simpson R B

出版信息

Nucleic Acids Res. 1980 Feb 25;8(4):759-66.

PMID:6253923
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327308/
Abstract

The cyclic AMP receptor protein (CRP) stimulates transcription of the lactose operon by binding to the lac promoter. I have identified those 5-positions of thymines in the promoter that lie close to bound CRP. Although ultraviolet irradiation of DNA with 5-bromouracil substituted in place of thymine normally cleaves the DNA at the bromouracils, a protein bound to the DNA can perturb these cleavages at those locations at which the protein lies close to the bromine. The contacts inferred from this photochemical probe and the results of nucleolytic attack of this complex by exonuclease III support a model where the cyclic AMP receptor protein binds to the promoter making symmetrical contacts with one face of the double helix and then stimulates transcription through contacts with RNA polymerase.

摘要

环磷酸腺苷受体蛋白（CRP）通过与乳糖操纵子启动子结合来刺激其转录。我已经确定了启动子中靠近结合的CRP的胸腺嘧啶的那些5位。尽管用5-溴尿嘧啶取代胸腺嘧啶对DNA进行紫外线照射通常会在溴尿嘧啶处切割DNA，但与DNA结合的蛋白质会在蛋白质靠近溴的那些位置干扰这些切割。从这种光化学探针推断出的接触以及核酸外切酶III对该复合物的核酸裂解攻击结果支持了一个模型，即环磷酸腺苷受体蛋白与启动子结合，与双螺旋的一个面进行对称接触，然后通过与RNA聚合酶的接触来刺激转录。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/239b/327308/8a5dbe0e96fd/nar00421-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/239b/327308/1e5597037bff/nar00421-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/239b/327308/8a5dbe0e96fd/nar00421-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/239b/327308/1e5597037bff/nar00421-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/239b/327308/8a5dbe0e96fd/nar00421-0065-a.jpg

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Cyclic AMP receptor proteins interacts with lactose operator DNA.环磷酸腺苷受体蛋白与乳糖操纵基因DNA相互作用。
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本文引用的文献

1
The lesions produced by ultraviolet light in DNA containing 5-bromouracil.紫外线在含有5-溴尿嘧啶的DNA中产生的损伤。
Q Rev Biophys. 1973 May;6(2):201-46. doi: 10.1017/s0033583500001141.
2
Lac operator analogues: bromodeoxyuridine substitution in the lac operator affects the rate of dissociation of the lac repressor.乳糖操纵子类似物：乳糖操纵子中的溴脱氧尿苷取代影响乳糖阻遏物的解离速率。
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2574-6. doi: 10.1073/pnas.69.9.2574.
3
The assembly of newly replicated DNA into chromatin.新复制的DNA组装成染色质。
大肠杆菌cAMP受体蛋白在乳糖和半乳糖启动子上结合位点的比较。
EMBO J. 1983;2(2):217-22. doi: 10.1002/j.1460-2075.1983.tb01408.x.
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Organization and expression of the Escherichia coli K-12 dad operon encoding the smaller subunit of D-amino acid dehydrogenase and the catabolic alanine racemase.编码D-氨基酸脱氢酶较小亚基和分解代谢丙氨酸消旋酶的大肠杆菌K-12 dad操纵子的组织与表达
J Bacteriol. 1994 Mar;176(5):1500-10. doi: 10.1128/jb.176.5.1500-1510.1994.
5
Repression and activation of transcription by Gal and Lac repressors: involvement of alpha subunit of RNA polymerase.半乳糖和乳糖阻遏物对转录的抑制与激活：RNA聚合酶α亚基的参与
EMBO J. 1995 Sep 15;14(18):4523-9. doi: 10.1002/j.1460-2075.1995.tb00131.x.
6
The catabolite-sensitive promoter for the chloramphenicol acetyl transferase gene is preceded by two binding sites for the catabolite gene activator protein.氯霉素乙酰转移酶基因的分解代谢物敏感型启动子之前有两个分解代谢物基因激活蛋白的结合位点。
J Bacteriol. 1982 Apr;150(1):312-8. doi: 10.1128/jb.150.1.312-318.1982.
7
Molecuar model of the DNA interaction site for the cyclic AMP receptor protein.环磷酸腺苷受体蛋白的DNA相互作用位点的分子模型。
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2213-7. doi: 10.1073/pnas.78.4.2213.
8
A model for catabolite activator protein binding to supercoiled DNA.一种分解代谢物激活蛋白与超螺旋DNA结合的模型。
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5263-7. doi: 10.1073/pnas.79.17.5263.
9
Molecular basis of DNA sequence recognition by the catabolite gene activator protein: detailed inferences from three mutations that alter DNA sequence specificity.分解代谢物基因激活蛋白识别DNA序列的分子基础：来自三个改变DNA序列特异性的突变的详细推断
Proc Natl Acad Sci U S A. 1984 Dec;81(23):7274-8. doi: 10.1073/pnas.81.23.7274.
10
A model for the non-specific binding of catabolite gene activator protein to DNA.一种分解代谢基因激活蛋白与DNA非特异性结合的模型。
Nucleic Acids Res. 1984 Nov 26;12(22):8475-87. doi: 10.1093/nar/12.22.8475.
Cold Spring Harb Symp Quant Biol. 1974;38:247-56. doi: 10.1101/sqb.1974.038.01.028.
4
Photochemical attachment of lac repressor to bromodeoxyuridine-substituted lac operator by ultraviolet radiation.通过紫外线辐射使乳糖阻遏物光化学附着于溴脱氧尿苷取代的乳糖操纵基因。
Proc Natl Acad Sci U S A. 1974 Mar;71(3):947-51. doi: 10.1073/pnas.71.3.947.
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The binding of cyclic adenosine monophosphate receptor to deoxyribonucleic acid.
J Biol Chem. 1972 Jul 10;247(13):4264-9.
6
Role of cyclic adenosine 3',5'-monophosphate and the cyclic adenosine 3',5'-monophosphate receptor protein in the initiation of lac transcription.环磷酸腺苷及环磷酸腺苷受体蛋白在乳糖转录起始中的作用。
J Biol Chem. 1971 Dec 10;246(23):7343-8.
7
Mechanism of initiation and repression of in vitro transcription of the lac operon of Escherichia coli.大肠杆菌乳糖操纵子体外转录的起始与抑制机制
Proc Natl Acad Sci U S A. 1971 Aug;68(8):1828-32. doi: 10.1073/pnas.68.8.1828.
8
Mechanism of activation of catabolite-sensitive genes: a positive control system.分解代谢物敏感基因的激活机制：一种正控制系统。
Proc Natl Acad Sci U S A. 1970 May;66(1):104-10. doi: 10.1073/pnas.66.1.104.
9
Cyclic AMP receptor protein of E. coli: its role in the synthesis of inducible enzymes.大肠杆菌的环磷酸腺苷受体蛋白：其在诱导酶合成中的作用。
Proc Natl Acad Sci U S A. 1970 Jun;66(2):480-7. doi: 10.1073/pnas.66.2.480.
10
Specific binding of CAP factor to lac promoter DNA.CAP因子与乳糖操纵子启动子DNA的特异性结合。
Nature. 1975 Aug 21;256(5519):672-4. doi: 10.1038/256672a0.