光合细菌含有细胞色素c1吗？
Do photosynthetic bacteria contain cytochrome c1?
作者信息
Wood P M
出版信息
Biochem J. 1980 Sep 1;189(3):385-91. doi: 10.1042/bj1890385.
Abstract
A method is described for characterizing, c-type cytochromes in bacterial membrane preparations according to molecular weight on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. Applied to the photosynthetic bacterium Rhodopseudomonas sphaeroides this technique is used, together with spectroscopic measurements, to demonstrate that a membrane-bound cytochrome c of mol.wt. 30000 is active in photosynthetic electron transport in addition to the well-known soluble cytochrome, cytochrome c2. The membrane cytochrome has a midpoint potential (E'0) at pH 7 of +290 mV, as compared with +360 mV for purified cytochrome c2. Its alpha-band has a peak near 552 nm, as compared with 550 nm for cytochrome c2. Evidence is presented that chromatophores contain roughly equal amounts of the two cytochromes.
摘要
本文描述了一种根据十二烷基硫酸钠/聚丙烯酰胺凝胶电泳上的分子量对细菌膜制剂中的c型细胞色素进行表征的方法。将该技术应用于光合细菌球形红假单胞菌，结合光谱测量，证明分子量为30000的膜结合细胞色素c除了众所周知的可溶性细胞色素细胞色素c2外，在光合电子传递中也具有活性。膜细胞色素在pH 7时的中点电位（E'0）为+290 mV，而纯化的细胞色素c2为+360 mV。其α带在552 nm附近有一个峰值，而细胞色素c2为550 nm。有证据表明，载色体中两种细胞色素的含量大致相等。
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