Montagnon B J, Nicolas A J, Fanget B, Peyron L
Dev Biol Stand. 1981;47:151-5.
Substitution of Primary Monkey Kidney (PMK) Cells by Monkey Cell Lines (MCL) for detection of residual live virus in poliovaccine control has been considered for various reasons. The sensitivity of VERO Cells versus PMK cells has been investigated. This study was done with poliovirus during and just after formalin inactivation. The amount of residual live virus was tested by TCID50 end-point titrations in roller-tubes and small bottles at different stages in the inactivation process. Just before or at the end of inactivation, a 1 000 doses vaccine equivalent volume was tested on Roux bottles. Thus far the results show that VERO cells seem to be less sensitive than PMK cells, especially for type 3 virus. Additional studies will be needed to investigate whether the sensitivity of the VERO cells can be improved to detect residual live poliovirus during and after inactivation with the same sensitivity as PMK cells.
出于多种原因，人们考虑用猴细胞系（MCL）替代原代猴肾（PMK）细胞来检测脊髓灰质炎疫苗对照中的残留活病毒。已对VERO细胞与PMK细胞的敏感性进行了研究。本研究是在脊髓灰质炎病毒福尔马林灭活期间及灭活后不久进行的。通过在滚瓶和小瓶中进行TCID50终点滴定，在灭活过程的不同阶段检测残留活病毒的量。在灭活前或灭活结束时，对相当于1000剂疫苗的体积在鲁氏瓶上进行检测。到目前为止，结果表明VERO细胞似乎比PMK细胞敏感性低，尤其是对3型病毒。还需要进一步研究，以探讨VERO细胞的敏感性是否能够提高，从而在灭活期间及灭活后以与PMK细胞相同的敏感性检测残留的脊髓灰质炎活病毒。
