Ménetrat J L, Chaniot S, Lefrançois S, Canale A, Chippaux A
Dev Biol Stand. 1981;47:163-7.
The purpose of this study is to know if it is possible to prepare inactivated poliomyelitis vaccines on Vero cell culture. In the cloned cell line used to prepare the vaccines, we have looked for an eventual toxic effect by different system tests. The cells between the 148e and 151e passage used to prepare the vaccines were inoculated into: - suckling mice, rabbits and guinea pigs - embryonated eggs. The supernatant fluid of the cells was inoculated into: - Vero cells - Primary rabbit kidney cells - Primary monkey kidney cells. We have also looked for an eventual tumorigenic effect on immunodepressed mice with an antithymocytic serum. The results of the assays are presented and discussed.
本研究的目的是了解是否有可能在Vero细胞培养物上制备灭活脊髓灰质炎疫苗。在用于制备疫苗的克隆细胞系中，我们通过不同的系统测试寻找可能的毒性作用。将用于制备疫苗的第148代至151代细胞接种到：- 乳鼠、兔子和豚鼠 - 鸡胚中。细胞的上清液接种到：- Vero细胞 - 原代兔肾细胞 - 原代猴肾细胞中。我们还研究了用抗胸腺细胞血清对免疫抑制小鼠的潜在致瘤作用。给出并讨论了测定结果。
