Horodniceanu F, Crainic R, Barme M
Dev Biol Stand. 1981;47:35-9.
By using 3H-T labeled HeLa cell DNA it was possible to evaluate the quantity and the state of substrate DNA present in crude and purified killed poliovaccine. The results showed that 1.7 x 10(5) pg/ml of DNA is present in crude vaccine while in purified vaccine this product can not be detected (the limit of the detection method was 1.1 x 10(2) pg/ml). The theoretical problems of the risk of the formation of poliovirus pseudotypes and the potential contamination of the vaccine with a type C retrovirus are examined.
通过使用3H-T标记的HeLa细胞DNA，可以评估粗制和纯化的灭活脊髓灰质炎疫苗中底物DNA的数量和状态。结果表明，粗制疫苗中存在1.7×10⁵ pg/ml的DNA，而在纯化疫苗中无法检测到该产物（检测方法的极限为1.1×10² pg/ml）。研究了脊髓灰质炎病毒假型形成风险以及疫苗被C型逆转录病毒潜在污染的理论问题。
