Yamaguchi N, Yoshimatsu K, Toyoshima S, Osawa T
J Immunol. 1981 Jun;126(6):2290-5.
A mitogenic substance, which stimulates murine and human B lymphocytes, has been isolated from the seeds of Ulex europeus. The m.w. of this mitogen was estimated to be approximately 100,000 by sodium dodecyl sulfate disc gel electrophoresis. Under physiologic conditions Ulex mitogen was in the form of a polymer. The isolated mitogen was found to contain 80% carbohydrate, in which glucose, galactose, and rhamnose were the predominant sugars. The carbohydrate portion of the mitogen could be responsible for the mitogenic activity, since the activity was destroyed by periodate treatment but not by protease digestion. It is unlikely that the mitogenic activity is due to the contamination by lipopolysaccharide (LPS), because Ulex mitogen strongly activated spleen cells from C3H/HeJ mice and did not contain a detectable amount of fatty acid. Spleen cells from nude mice, T-depleted spleen cells from conventional mice, or T-depleted spleen cells from conventional mice, or T-depleted lymphocytes from human peripheral blood responded as strongly as lymphocytes (T + B) from normal origin as measured by 3H-thymidine incorporation. It seems that the mitogenic effect is primarily on B cells. Ulex mitogen also induced the increase of immunoglobulin synthesis in murine and human B cells.
从欧洲荆豆种子中分离出一种能刺激小鼠和人B淋巴细胞的促有丝分裂物质。通过十二烷基硫酸钠圆盘凝胶电泳估计该促有丝分裂原的分子量约为100,000。在生理条件下，欧洲荆豆促有丝分裂原呈聚合物形式。发现分离出的促有丝分裂原含有80%的碳水化合物，其中葡萄糖、半乳糖和鼠李糖是主要糖类。促有丝分裂原的碳水化合物部分可能是其促有丝分裂活性的原因，因为该活性经高碘酸盐处理后被破坏，但蛋白酶消化后未被破坏。促有丝分裂活性不太可能是由于脂多糖（LPS）污染，因为欧洲荆豆促有丝分裂原能强烈激活C3H/HeJ小鼠的脾细胞，且不含可检测量的脂肪酸。通过3H-胸腺嘧啶核苷掺入法测定，裸鼠的脾细胞、常规小鼠的T细胞缺失脾细胞或人外周血的T细胞缺失淋巴细胞与正常来源的淋巴细胞（T + B）反应一样强烈。促有丝分裂作用似乎主要作用于B细胞。欧洲荆豆促有丝分裂原还能诱导小鼠和人B细胞中免疫球蛋白合成增加。
