Engel D, Clagett J, Page R, Williams B
J Immunol. 1977 Apr;118(4):1466-71.
Actinomyces viscosus homogenate (AVIS) contins substance(s) which cause spleen cells from conventional and germfree mice to undergo increased DNA synthesis. This mitogenic effect is primarily on B cells since spleen cells from nude mice or T-depleted spleen cells from conventional mice respond as strongly as conventional (T + B) spleen cells. Mouse thymocytes do not respond mitogenically to AVIS. It is unlikely that the mitogenic acitivity is due to the presence of LPS, since A. viscosus is Gram-positive and is not known to have an LPS cell wall component. Also, AVIS is not inactivated by polymyxin B, as are some preparations of LPS, and C3H/HeJ mouse splenocytes respond strongly to AVIS but not to LPS. The activity is heat stable, is not lost upon dialysis, and is not affected by lysozyme. Mitogenic activity is partially lost when AVIS is digested with nonspecific bacterial protease or treated with metaperiodate. Sodium hydroxide treatment completely abolishes mitogenic activity. Actinomycotic lesions are characterized by a long-tern inflammatory response involving a dense plasma cell infiltrate. We suggest that B cell mitogens form Actinomyces may play a role in the elicitation of the plasma cell component of these lesions.
粘性放线菌匀浆（AVIS）含有能使常规饲养小鼠和无菌小鼠的脾细胞DNA合成增加的物质。这种促有丝分裂作用主要作用于B细胞，因为裸鼠的脾细胞或常规饲养小鼠经T细胞去除后的脾细胞与常规（T + B）脾细胞的反应一样强烈。小鼠胸腺细胞对AVIS无促有丝分裂反应。这种促有丝分裂活性不太可能是由于脂多糖（LPS）的存在，因为粘性放线菌是革兰氏阳性菌，且已知其没有LPS细胞壁成分。此外，AVIS不像某些LPS制剂那样会被多粘菌素B灭活，并且C3H/HeJ小鼠脾细胞对AVIS反应强烈，但对LPS无反应。该活性对热稳定，透析后不失活，且不受溶菌酶影响。用非特异性细菌蛋白酶消化AVIS或用过碘酸盐处理后，促有丝分裂活性部分丧失。用氢氧化钠处理则完全消除促有丝分裂活性。放线菌病病变的特征是长期的炎症反应，伴有密集的浆细胞浸润。我们认为，来自放线菌的B细胞促有丝分裂原可能在引发这些病变的浆细胞成分中起作用。
