[大鼠肝脏线粒体DNA复制起点的定位与限制酶图谱分析]
[Localization and restriction mapping of the origin of replication of rat liver mitochondrial DNA].
作者信息
Tomarev S I, Zakhar'ev V M, Zl'darov M A
出版信息
Biokhimiia. 1980 Oct;45(10):1909-12.
PMID:6263363
Abstract
Based on plasmid pCV II, a recombinant comprising the origin of replication of rat liver mitochondrial DNA was obtained. A detailed restriction map of the region based on restrictases Hae III, Hpa II and Hind II was developed. The origin of mitochondrial DNA replication is localized in one of Hind II fragments.
摘要
基于质粒pCV II，获得了一个包含大鼠肝线粒体DNA复制起点的重组体。绘制了基于限制性内切酶Hae III、Hpa II和Hind II的该区域的详细限制性图谱。线粒体DNA复制起点定位于Hind II片段之一中。
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