Lisova N E, Fedenko E P
Biokhimiia. 1981 Mar;46(3):520-4.
The phosphodiesterase (PDE) activity of adenosine-3':5'-monophosphate was detected in the cells of tubercular bacteria of Rhizobium lupini and Rhizobium japonicum. The specific activity of three Rhizobium forms, e.g. bacteroids from lupine root tubercles, free-nitrogen-fixing culture and vegetative cells grown on a mannitol--yeast agar, were compared. In the bacteroids PDE is represented both by soluble and membrane-bound forms. The optimal enzyme activity is revealed in an alkaline medium, whereas the curve of PDE activity dependence on pH has a broad maximum. PDE is inhibited by methylxanthines, the inhibiting effect being stronger than that of theophylline.
在羽扇豆根瘤菌和日本根瘤菌的结核细菌细胞中检测到了3':5'-环磷酸腺苷的磷酸二酯酶（PDE）活性。比较了三种根瘤菌形式的比活性，例如羽扇豆根瘤中的类菌体、游离固氮培养物以及在甘露醇 - 酵母琼脂上生长的营养细胞。在类菌体中，PDE以可溶性和膜结合形式存在。在碱性介质中显示出最佳酶活性，而PDE活性对pH的依赖性曲线有一个较宽的最大值。PDE受到甲基黄嘌呤的抑制，其抑制作用比茶碱更强。
