Büültjens T E, Macnab J C
Cancer Res. 1981 Jun;41(6):2540-7.
Four independent isolated lines transformed by ts mutant or sheared DNA of herpes simplex virus types 1 and 2 and one tumor cell line were characterized according to their behavior in assays of aggregation, growth in low serum, doubling times, saturation density, agglutination by lectins, uptake of metabolites, and production of plasminogen activator. The criteria used to select the transformed cells used in this study were the formation of altered foci of cells followed by the formation of a continuous cell line, the continuing expression of herpes simplex virus-specific antigens, and the tumorigenicity of the transformed cell lines. The results indicate that, whereas the one herpes simplex virus type 2-transformed cell line investigated showed many of the transformation parameters accepted as typical of cells transformed by the papovaviruses, the three herpes simplex virus type 1-transformed cell lines do not share or express many of these transformation parameters.
根据单纯疱疹病毒1型和2型的温度敏感突变体或剪切DNA转化得到的四个独立分离株系以及一个肿瘤细胞系，在聚集试验、低血清培养生长、倍增时间、饱和密度、凝集素凝集、代谢物摄取和纤溶酶原激活物产生等方面的表现进行了表征。本研究中用于选择转化细胞的标准是形成细胞改变灶，随后形成连续细胞系、单纯疱疹病毒特异性抗原的持续表达以及转化细胞系的致瘤性。结果表明，虽然所研究的一个单纯疱疹病毒2型转化细胞系表现出许多被认为是乳头瘤病毒转化细胞典型的转化参数，但三个单纯疱疹病毒1型转化细胞系并不具备或表达其中许多转化参数。
