Kasuga M, Van Obberghen E, Nissley S P, Rechler M M
J Biol Chem. 1981 Jun 10;256(11):5305-8.
The structure of receptors for insulin-like growth factors in rat liver plasma membranes and the BRL 3A2 rat liver cell line has been examined by chemical cross-linking with disuccinimidyl suberate and sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing and nonreducing conditions. Two receptor subtypes have been identified: (i) 125I multiplication-stimulating activity cross-linked to liver membranes or intact cells appeared in a complex of Mr = 260,000 (reduced) and 220,000 (nonreduced) and (ii) 125I-insulin-like growth factor I cross-linked to BRL 3A2 cells appeared predominantly in two bands of Mr greater than 300,000 without disulfide reduction and in a Mr = 130,000 complex following reduction. The two subtypes of insulin-like growth factor receptors identified by structural analysis correspond to previously observed differences in their specificity for insulin and insulin-like growth factors.
通过在还原和非还原条件下用辛二酸二琥珀酰亚胺酯进行化学交联以及十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳，对大鼠肝细胞膜和BRL 3A2大鼠肝细胞系中胰岛素样生长因子受体的结构进行了研究。已鉴定出两种受体亚型：(i) 与肝细胞膜或完整细胞交联的125I增殖刺激活性出现在Mr = 260,000（还原）和220,000（非还原）的复合物中；(ii) 与BRL 3A2细胞交联的125I - 胰岛素样生长因子I在未进行二硫键还原时主要出现在两条Mr大于300,000的条带中，还原后出现在Mr = 130,000的复合物中。通过结构分析鉴定出的胰岛素样生长因子受体的两种亚型与先前观察到的它们对胰岛素和胰岛素样生长因子的特异性差异相对应。
