Veltri R W, Kikta V A, Wainwright W H, Sprinkle P M
J Immunol. 1981 Jul;127(1):320-8.
Our laboratory has previously reported the isolation of a serum blocking factor (SBF) from infectious mononucleosis (IM) patients. The SBF has been purified by a combination of Sephadex QAE-50 ion exchange and Sephadex G-200 molecular sieve chromatography. This material was found to be devoid of soluble immune complexes, and immunochemically and biochemically was characterized as IgG and, hence, termed SBF-IgG. The SBF-IgG was shown to significantly (alpha = 0.05) suppress antigen specific (Influenza A-1[H1N1]) in vitro lymphocyte stimulation (LS) as well as leukocyte migration-inhibition (LMI) reactivity. Also, the SBF-IgG significantly suppressed the in vitro.LS response to phytohemagglutinin. In addition, the SBF-IgG when bound to normal donor lymphocytes significantly reduced the high affinity E-rosette (HAR) reactivity at 29 degrees C. A purified T lymphocyte subpopulation of normal donor lymphocytes specifically bound SBF-IgG, and the latter could be r covered using glycine-HCI. It appears that SBF-IgG is a nonspecific antibody; it binds neither lymphokines nor specific antigen, but apparently elicits its in vitro vitro cell-mediated suppressive effect at the level of the T lymphocytes.
我们实验室先前曾报道过从传染性单核细胞增多症（IM）患者中分离出一种血清封闭因子（SBF）。通过葡聚糖QAE - 50离子交换和葡聚糖G - 200分子筛层析相结合的方法对SBF进行了纯化。发现该物质不含可溶性免疫复合物，经免疫化学和生物化学鉴定为IgG，因此称为SBF - IgG。结果表明，SBF - IgG能显著（α = 0.05）抑制体外抗原特异性（甲型流感病毒1型[H1N1]）淋巴细胞刺激（LS）以及白细胞游走抑制（LMI）反应性。此外，SBF - IgG还能显著抑制体外对植物血凝素的LS反应。另外，当SBF - IgG与正常供体淋巴细胞结合时，在29℃下能显著降低高亲和力E - 玫瑰花结（HAR）反应性。正常供体淋巴细胞的纯化T淋巴细胞亚群能特异性结合SBF - IgG，且可用甘氨酸 - 盐酸将其洗脱。似乎SBF - IgG是一种非特异性抗体；它既不结合淋巴因子也不结合特异性抗原，但显然在T淋巴细胞水平引发其体外细胞介导的抑制作用。
