Sandri-Goldin R M, Levine M, Glorioso J C
J Virol. 1981 Apr;38(1):41-9. doi: 10.1128/JVI.38.1.41-49.1981.
A procedure was developed for inducing mutations in isolated restriction enzyme fragments of herpes simplex virus type 1 (HSV-1) DNA with nitrous acid. The mutations were then transferred to the viral genome by genetic recombination during cotransfection of rabbit kidney cells with the mutagenized fragments and intact HSV-1 DNA. The HpaI restriction enzyme fragments LD, B, LG, I, and J were mutagenized. Temperature-sensitive mutants were found at frequencies of 1 to 5% among the progeny of the transfections. Syncytial mutants also were found at high frequency when fragment B or LD was used for mutagenesis. Fifteen of these mutants, 11 temperature sensitive and 4 syncytial, were used for further studies, including complementation analysis, DNA synthesis, and marker rescue. Marker rescue data presented here and in the accompanying publication (A. L. Goldin, R. M. Sandri-Goldin, M. Levine, and J. C. Glorioso, J. Virol. 38: 50-58, 1981) confirm the map position of some of the newly isolated mutants.
已开发出一种用亚硝酸诱导单纯疱疹病毒1型（HSV-1）DNA的分离限制性酶切片段发生突变的方法。然后，在将诱变片段与完整的HSV-1 DNA共转染兔肾细胞的过程中，通过基因重组将这些突变转移到病毒基因组中。对HpaI限制性酶切片段LD、B、LG、I和J进行了诱变。在转染后代中，温度敏感突变体的出现频率为1%至5%。当使用片段B或LD进行诱变时，多核体突变体也以高频率出现。这些突变体中的15个，11个温度敏感型和4个多核体型，用于进一步研究，包括互补分析、DNA合成和标记拯救。此处及随附出版物（A. L. Goldin、R. M. Sandri-Goldin、M. Levine和J. C. Glorioso，《病毒学杂志》38: 50 - 58, 1981）中呈现的标记拯救数据证实了一些新分离突变体的图谱位置。
