Adhya S, Basu S, Sarkar P, Maitra U
Proc Natl Acad Sci U S A. 1981 Jan;78(1):147-51. doi: 10.1073/pnas.78.1.147.
The major promoters for bacteriophage T3 RNA polymerase on the T3 genome have been mapped by DNA.RNA filter hybridization. One promoter is located in a 300-base-pair Hpa I restriction fragment near the genetic "left" end of T3 DNA. The sequence in the vicinity of the major initiation site of transcription in this region has been determined. A part of the (-)strand sequence is 5' T-A-T-T-T-A-C-C-C-T-C-A-C-T-A-A-A-G-+1 G-G-A-A-U 3'. Comparison of this sequence with the prototype 23-base-pair promoter sequence for bacteriophage T7 RNA polymerase shows a striking pattern of homology and divergence. Between positions -9 and +4, the sequences are virtually identical, whereas between positions -17 and -10, the sequences are quite different. It is postulated that these sequence subsets may perform different functions in transcription initiation by the phage RNA polymerases.
通过DNA-RNA滤膜杂交技术，已绘制出噬菌体T3基因组上T3 RNA聚合酶的主要启动子图谱。一个启动子位于靠近T3 DNA遗传“左端”的一个300碱基对的Hpa I限制性片段中。已确定该区域转录主要起始位点附近的序列。（-）链序列的一部分是5'T-A-T-T-T-A-C-C-C-T-C-A-C-T-A-A-A-G-+1 G-G-A-A-U 3'。将该序列与噬菌体T7 RNA聚合酶的23碱基对启动子原型序列进行比较，发现了显著的同源性和差异模式。在-9至+4位之间，序列几乎相同，而在-17至-10位之间，序列则有很大差异。据推测，这些序列子集在噬菌体RNA聚合酶的转录起始过程中可能发挥不同的功能。
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