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噬菌体T3诱导的聚合酶以T3 DNA模板（大肠杆菌-三磷酸鸟苷末端-纯化的聚合酶）起始、释放和重新起始RNA链。

Initiation, release, and reinitiation of RNA chains by bacteriophage-T3-induced polymerase from T3 DNA templates (E. coli-guanosine triphosphate terminus-purified polymerase).

作者信息

Maitra U, Huang H H

出版信息

Proc Natl Acad Sci U S A. 1972 Jan;69(1):55-9. doi: 10.1073/pnas.69.1.55.

DOI:10.1073/pnas.69.1.55
PMID:4550510
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC427543/
Abstract

Bacteriophage T3-induced RNA polymerase, upon copying its specific template, native T3 DNA, initiates RNA chains only with GTP. Denaturation of the DNA results in loss of template specificity for the polymerase. With denatured T3 DNA as template, T3 polymerase initiates RNA chains with both ATP and GTP, and the average length of the resulting RNA chains is markedly reduced. Studies of the polymerase reaction with native T3 DNA in vitro show that T3 polymerase is able to terminate RNA synthesis with the release of RNA chains from the template DNA. Polymerase is also released in the process and, acting catalytically, reinitiates new RNA chains. Many moles of RNA chains are thus formed per mole of polymerase added to the reaction mixture.

摘要

噬菌体T3诱导的RNA聚合酶在复制其特定模板——天然T3 DNA时，仅以GTP起始RNA链。DNA变性会导致聚合酶失去模板特异性。以变性的T3 DNA为模板时，T3聚合酶会以ATP和GTP起始RNA链，且所产生的RNA链的平均长度会显著缩短。体外对天然T3 DNA进行的聚合酶反应研究表明，T3聚合酶能够通过从模板DNA释放RNA链来终止RNA合成。聚合酶在此过程中也会被释放出来，并发挥催化作用重新起始新的RNA链。因此，每向反应混合物中添加一摩尔聚合酶，就会形成许多摩尔的RNA链。

相似文献

1
Initiation, release, and reinitiation of RNA chains by bacteriophage-T3-induced polymerase from T3 DNA templates (E. coli-guanosine triphosphate terminus-purified polymerase).噬菌体T3诱导的聚合酶以T3 DNA模板（大肠杆菌-三磷酸鸟苷末端-纯化的聚合酶）起始、释放和重新起始RNA链。
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引用本文的文献

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Location, function, and nucleotide sequence of a promoter for bacteriophage T3 RNA polymerase.噬菌体T3 RNA聚合酶启动子的定位、功能及核苷酸序列
Proc Natl Acad Sci U S A. 1981 Jan;78(1):147-51. doi: 10.1073/pnas.78.1.147.
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本文引用的文献

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FORMATION AND PROPERTIES OF RNA-DNA COMPLEXES.RNA-DNA复合物的形成与特性
J Mol Biol. 1964 Jul;9:125-42. doi: 10.1016/s0022-2836(64)80095-4.
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The role of DNA in RNA synthesis, IX. Nucleoside triphosphate termini in RNA polymerase products.DNA在RNA合成中的作用，IX. RNA聚合酶产物中的核苷三磷酸末端。
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New RNA polymerase from Escherichia coli infected with bacteriophage T7.来自被噬菌体T7感染的大肠杆菌的新型RNA聚合酶。
Nature. 1970 Oct 17;228(5268):227-31. doi: 10.1038/228227a0.
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Termination factor for RNA synthesis.RNA合成的终止因子。
Nature. 1969 Dec 20;224(5225):1168-74. doi: 10.1038/2241168a0.
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DNA-dependent synthesis of RNA by Escherichia coli RNA polymerase: release and reinitiation of RNA chains from DNA templates.大肠杆菌RNA聚合酶依赖DNA的RNA合成：RNA链从DNA模板上的释放与重新起始
Proc Natl Acad Sci U S A. 1969 Oct;64(2):779-86. doi: 10.1073/pnas.64.2.779.
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The role of deoxyribonucleic acid in ribonucleic acid synthesis. XIV. A study of the initiation of ribonucleic acid synthesis.脱氧核糖核酸在核糖核酸合成中的作用。十四。核糖核酸合成起始的研究。
J Biol Chem. 1967 Nov 10;242(21):4908-18.
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The role of deoxyribonucleic acid in ribonucleic acid synthesis. 13. Modified purification procedure and additional properties of ribonucleic acid polymerase from Escherichia coli W.脱氧核糖核酸在核糖核酸合成中的作用。13. 大肠杆菌W核糖核酸聚合酶的改良纯化程序及其他特性。
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