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Isolation and sequence determination of 5'-terminal oligonucleotide fragments of RNA transcripts synthesized by bacteriophage T3-induced RNA polymerase from T3 DNA.

作者信息

Maitra U, Jelinek W, Yudelevich A, Majumder H K, Guha A

出版信息

Proc Natl Acad Sci U S A. 1980 Jul;77(7):3908-11. doi: 10.1073/pnas.77.7.3908.

DOI:10.1073/pnas.77.7.3908
PMID:6933443
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC349736/
Abstract

The nucleotide sequence of the 5'-terminal oligonucleotides produced by pancreatic RNase digestion of bacteriophage T3 RNA polymerase (EC 2.7.7.6) transcripts of T3 DNA has been determined. The sequence determination is based upon a simple isolation procedure for the 5'-terminal oligonucleotides. This procedure involves treatment of pancreatic RNase digests of alpha 32P-labeled T3 RNA polymerase transcripts with bovine brain exoribonuclease to remove oligonucleotides with free 5'-hydroxyl termini and then chromatographing the products on hydroxylapatite to resolve the remaining oligonucleotides having 5'-phosphate termini. By application of standard two-dimensional separation and sequence techniques, the major 5'-end sequences deduced were pppGpGpGpApGpApGpApY(Y = pyrimidine nucleoside) and pppGpGpGpApGpApCp. In addition, the sequences of other minor 5'-terminal oligonucleotides observed on homochromatograms were also determined. The sequences of these 5'-oligonucleotides were pppGpGpGpApApCpY, pppGpGpGpApApUpY, pppGpGp(2-4 Gp, 2-3 ApGp)..., and pppGpGpGp.... These results demonstrate that T3 phage-induced RNA polymerase possesses a high degree of specificity in the initiation of RNA chains.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/496f/349736/70a10f852077/pnas00494-0201-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/496f/349736/debaff699c99/pnas00494-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/496f/349736/3446c00728f2/pnas00494-0201-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/496f/349736/70a10f852077/pnas00494-0201-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/496f/349736/debaff699c99/pnas00494-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/496f/349736/3446c00728f2/pnas00494-0201-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/496f/349736/70a10f852077/pnas00494-0201-c.jpg

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引用本文的文献

1
Location, function, and nucleotide sequence of a promoter for bacteriophage T3 RNA polymerase.噬菌体T3 RNA聚合酶启动子的定位、功能及核苷酸序列
Proc Natl Acad Sci U S A. 1981 Jan;78(1):147-51. doi: 10.1073/pnas.78.1.147.

本文引用的文献

1
Recognition and initiation site for four late promoters of phage T7 is a 22-base pair DNA sequence.噬菌体T7四个晚期启动子的识别和起始位点是一段22个碱基对的DNA序列。
Nature. 1979 Jul 5;280(5717):35-9. doi: 10.1038/280035a0.
2
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.一种制备高比活度32-P标记三磷酸腺苷的简单方法。
Biochem J. 1964 Jan;90(1):147-9. doi: 10.1042/bj0900147.
3
Induction of a new RNA polymerase in Escherichia coli infected with bacteriophage T3.感染噬菌体T3的大肠杆菌中新RNA聚合酶的诱导。
Biochem Biophys Res Commun. 1971 Apr 16;43(2):443-50. doi: 10.1016/0006-291x(71)90773-x.
4
Different template specificities of phage T3 and T7 RNA polymerases.噬菌体T3和T7 RNA聚合酶不同的模板特异性。
Nat New Biol. 1971 Mar 17;230(11):94-6. doi: 10.1038/newbio230094a0.
5
Fidelity of in vitro transcription of T3 deoxyribonucleic acid by bacteriophage T3-induced ribonucleic acid polymerase and by Escherichia coli ribonucleic acid polymerase.噬菌体T3诱导的核糖核酸聚合酶和大肠杆菌核糖核酸聚合酶对T3脱氧核糖核酸的体外转录保真度
J Biol Chem. 1974 Nov 10;249(21):6901-9.
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Bacteriophage T7.噬菌体T7
Science. 1972 Apr 28;176(4033):367-76. doi: 10.1126/science.176.4033.367.
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In vitro transcription of T3 DNA by Escherichia coli and T3 polymerases.大肠杆菌和T3聚合酶对T3 DNA的体外转录
Virology. 1972 Apr;48(1):112-25. doi: 10.1016/0042-6822(72)90119-5.
8
Initiation, release, and reinitiation of RNA chains by bacteriophage-T3-induced polymerase from T3 DNA templates (E. coli-guanosine triphosphate terminus-purified polymerase).噬菌体T3诱导的聚合酶以T3 DNA模板（大肠杆菌-三磷酸鸟苷末端-纯化的聚合酶）起始、释放和重新起始RNA链。
Proc Natl Acad Sci U S A. 1972 Jan;69(1):55-9. doi: 10.1073/pnas.69.1.55.
9
A preliminary map of the major transcription units read by T7 RNA polymerase on the T7 and T3 bacteriophage chromosomes.T7 RNA聚合酶在T7和T3噬菌体染色体上读取的主要转录单元的初步图谱。
Proc Natl Acad Sci U S A. 1974 Mar;71(3):760-4. doi: 10.1073/pnas.71.3.760.
10
Specificity of ribonucleic acid chain initiation by bacteriophage T3-induced ribonucleic acid polymerase.噬菌体T3诱导的核糖核酸聚合酶引发核糖核酸链的特异性
J Biol Chem. 1974 Sep 25;249(18):5835-9.