转座子Tn5的切除依赖于反向重复序列，而不依赖于Tn5的转座酶功能。
Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5.
作者信息
Egner C, Berg D E
出版信息
Proc Natl Acad Sci U S A. 1981 Jan;78(1):459-63. doi: 10.1073/pnas.78.1.459.
Abstract
The excision of Tn5 from sites of insertion in the Escherichia coli genome was studied by examining the reversion of lac::Tn5 insertion mutations to lac+. We find that: (i) the frequency of excision depends on the site of Tn5 insertion, (ii) excision occurs efficiently in recA- cells, (iii) excision does not require a Tn5-encoded transposition function, and (iv) efficient excision requires the inverted repeats of Tn5. We propose that excision of Tn5 is similar to the formation of spontaneous deletions and occurs by slippage during DNA synthesis.
摘要
通过检测 lac::Tn5 插入突变回复为 lac+ 的情况，研究了 Tn5 从大肠杆菌基因组插入位点的切除。我们发现：（i）切除频率取决于 Tn5 的插入位点，（ii）切除在 recA- 细胞中高效发生，（iii）切除不需要 Tn5 编码的转座功能，并且（iv）高效切除需要 Tn5 的反向重复序列。我们提出 Tn5 的切除类似于自发缺失的形成，并且是在 DNA 合成过程中通过滑动发生的。
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Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5.转座子Tn5的切除依赖于反向重复序列，而不依赖于Tn5的转座酶功能。
Proc Natl Acad Sci U S A. 1981 Jan;78(1):459-63. doi: 10.1073/pnas.78.1.459.
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