Schlegel Susan, Genevaux Pierre, de Gier Jan-Willem
Department of Environmental Systems Science, ETH Zürich, 8092, Zürich, Switzerland.
Laboratoire de Microbiologie et de Génétique Moléculaires, Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, Toulouse, France.
Cell Mol Life Sci. 2017 Mar;74(5):891-908. doi: 10.1007/s00018-016-2371-2. Epub 2016 Oct 11.
Escherichia coli has been widely used for the production of recombinant proteins. To improve protein production yields in E. coli, directed engineering approaches have been commonly used. However, there are only few reported examples of the isolation of E. coli protein production strains using evolutionary approaches. Here, we first give an introduction to bacterial evolution and mutagenesis to set the stage for discussing how so far selection- and screening-based approaches have been used to isolate E. coli protein production strains. Finally, we discuss how evolutionary approaches may be used in the future to isolate E. coli strains with improved protein production characteristics.
大肠杆菌已被广泛用于重组蛋白的生产。为提高大肠杆菌中的蛋白产量，人们通常采用定向工程方法。然而，利用进化方法分离大肠杆菌蛋白生产菌株的报道实例却很少。在此，我们首先介绍细菌进化和诱变，为讨论迄今为止基于选择和筛选的方法如何用于分离大肠杆菌蛋白生产菌株奠定基础。最后，我们讨论未来如何利用进化方法分离具有改善蛋白生产特性的大肠杆菌菌株。
