Schirrmeier H, Heinrich H W
Arch Exp Veterinarmed. 1981;35(2):187-98.
A methodical account is given of possible applications of the immuno-fluorescence technique in the diagnosis of rotavirus infection of calf. The technique has proved suitable for routine checks for both its low input in terms of method and hardware and its potential of diagnostic information. The two latter methods are best applicable under routine conditions to testing of faeces-inoculated cell cultures as well as to the detection of rotavirus from faecal smears and frozen intestinal sections. In investigations on the dynamics of rotavirus development in cell cultures, the first freshly formed virus protein was detected six hours from inoculation. The time of one replication cycle was found to be between 16 and 18 hours, with several replication cycles running consecutively, when it comes to virus strains which have become adapted to the cell cultures concerned.
本文系统阐述了免疫荧光技术在诊断犊牛轮状病毒感染方面的可能应用。该技术已被证明适用于常规检查，因其在方法和硬件方面投入较低，且具有诊断信息潜力。后两种方法在常规条件下最适用于检测接种粪便的细胞培养物，以及从粪便涂片和冷冻肠切片中检测轮状病毒。在对细胞培养中轮状病毒发育动态的研究中，接种后6小时检测到首个新形成的病毒蛋白。对于已适应相关细胞培养的病毒株，发现一个复制周期的时间在16至18小时之间，且有几个复制周期连续进行。
