Niwa O, Sugahara T
Cancer Res. 1981 Aug;41(8):3253-9.
The effect of caffeine on the expression of murine endogenous virus in mouse cells induced by radiation and chemicals was studied. Postirradiation treatment of K-BALB cells with caffeine enhanced cell killing as well as the induction of xenotropic virus after ultraviolet light irradiation. The degree of enhancement for the virus induction was comparable to that for cell killing. On the other hand, colony-forming ability and the expression of xenotropic virus of K-BALB cells after X-irradiation were unaffected by caffeine. These data suggest a linear relationship between the degree of endogenous virus expression and the amount of lethal damages after irradiation. For induction by halogenated pyrimidines, a 24-hr incubation of AKR2B cells with caffeine after 5-iodo-2'-deoxyuridine treatment resulted in marked suppression of the expression of ecotropic virus. On the contrary, in K-BALB cells, caffeine exerted only a small effect on 5-iodo-2'-deoxyuridine-induced expression of ecotropic and xenotropic viruses. These results indicate that, although using the same inducing agent, the pathway of endogenous virus induction may be different for AKR2B cells and for K-BALB cells.
研究了咖啡因对辐射和化学物质诱导的小鼠细胞中鼠内源性病毒表达的影响。用咖啡因对K - BALB细胞进行辐照后处理，在紫外线照射后增强了细胞杀伤以及嗜异性病毒的诱导。病毒诱导的增强程度与细胞杀伤的增强程度相当。另一方面，X射线照射后K - BALB细胞的集落形成能力和嗜异性病毒的表达不受咖啡因影响。这些数据表明内源性病毒表达程度与辐照后致死损伤量之间存在线性关系。对于卤代嘧啶诱导，在5 - 碘 - 2'-脱氧尿苷处理后，将AKR2B细胞与咖啡因孵育24小时导致嗜亲性病毒表达明显受到抑制。相反，在K - BALB细胞中，咖啡因对5 - 碘 - 2'-脱氧尿苷诱导的嗜亲性和嗜异性病毒表达仅产生微小影响。这些结果表明，尽管使用相同的诱导剂，但AKR2B细胞和K - BALB细胞内源性病毒诱导途径可能不同。
