Barré-Sinoussi F, Montagnier L, Lidereau R, Sisman J, Wood J, Chermann J C
Ann Microbiol (Paris). 1979 Oct;130B(3):349-62.
In order to investigate the role of endogenous interferon in retrovirus production by infected or induced cells, the effect of two sera raised against mouse interferon has been tested on various C-type murine viruses. Addition of a highly potent anti-interferon serum to 3T3/IC cells chronically infected by the Moloney strain of MLV results in a considerable increase of virus production, as tested by reverse transcriptase assay. This effect is neutralized by an excess of exogenous interferon. The greatest effect of anti-interferon sera was obtained in the derepression of endogenous retroviruses: in K. BALB/c cells treated by IUDR, anti-interferon serum increases up to 50-fold the expression of the endogenous virus. The extinction of virus production which secondarily occurs after its induction by IUdR is likely to be caused by cellular endogenous interferon. The biological parameters of the viral agent produced in the presence of anti-interferon serum are those of the xenotropic endogenous virus.
为了研究内源性干扰素在受感染或诱导细胞产生逆转录病毒中的作用，已对两种针对小鼠干扰素的抗血清作用于各种C型鼠病毒的效果进行了测试。将高效抗干扰素血清添加到长期感染莫洛尼氏鼠白血病病毒（MLV）莫洛尼株的3T3/IC细胞中，通过逆转录酶测定法检测发现病毒产生显著增加。这种效应可被过量的外源性干扰素中和。抗干扰素血清在内源性逆转录病毒的去抑制方面效果最为显著：在用碘脱氧尿苷（IUDR）处理的K.BALB/c细胞中，抗干扰素血清可使内源性病毒的表达增加多达50倍。在用IUDR诱导病毒产生后随后发生的病毒产生消退可能是由细胞内源性干扰素引起的。在抗干扰素血清存在下产生的病毒因子的生物学参数是嗜异性内源性病毒的参数。
