Weygand-Durasevic I, Kruse T A, Clark B F
Eur J Biochem. 1981 May;116(1):59-65. doi: 10.1111/j.1432-1033.1981.tb05300.x.
The interactions of yeast tRNATyr, spin-labelled at position i6A-37 next to the anticodon, with EF-Tu . GTP and with Escherichia coli tRNAVal (which has a complementary anticodon) have been studied. The immobilization of the spin label upon ternary complex formation shows a conformational change of the anticodon region, although this part of tRNATyr is not in direct contact with the protein, as indicated by RNase T1 digestion. Upon anticodon-anticodon interaction, no conformational change of the anticodon loop of tRNATyr was observed.
对在反密码子旁的i6A - 37位置进行自旋标记的酵母tRNATyr与EF - Tu·GTP以及与大肠杆菌tRNAVal（其具有互补反密码子）之间的相互作用进行了研究。尽管如核糖核酸酶T1消化所示，tRNATyr的这部分不与蛋白质直接接触，但三元复合物形成时自旋标记的固定表明反密码子区域发生了构象变化。在反密码子 - 反密码子相互作用时，未观察到tRNATyr反密码子环的构象变化。
