Kemkhadze K Sh, Odintsov V B, Makhno V I, Semenkov Iu P, Kirillov S V
Mol Biol (Mosk). 1981 Jul-Aug;15(4):779-89.
Purified Phe-tRNAPhe revealed higher affinity to the donor (D) site of vacant 70S . poly(U) complex than to the acceptor (A) site, independent on Mg2+ concentration. As a result, in excess of ribosomes Phe-tRNAPhe binds exclusively to the D site. This was proved using the tests in the presence of tetracycline and puromycin. Preferential binding of Phe-tRNAPhe to the D site was used to measure equilibrium association constants of this interaction at different temperatures and Mg2+ concentrations. A large value of reaction enthalpy (ca. -26 Kcal/mole) was found.
纯化的苯丙氨酰 - tRNAphe对空载70S·聚（U）复合物的供体（D）位点显示出比对受体（A）位点更高的亲和力，且与Mg2 +浓度无关。因此，在过量核糖体存在的情况下，苯丙氨酰 - tRNAphe仅与D位点结合。这在四环素和嘌呤霉素存在下的测试中得到了证实。利用苯丙氨酰 - tRNAphe对D位点的优先结合来测量该相互作用在不同温度和Mg2 +浓度下的平衡缔合常数。发现反应焓值很大（约 - 26千卡/摩尔）。
