Azzarone B, Curatolo C, Carloni G, Doanti M B, Morasca L, Macieira-Coelho A
J Natl Cancer Inst. 1981 Jul;67(1):89-94.
Normal avian embryo fibroblasts induced retraction of a fibrin clot. The expression of high levels of this activity was associated with the exponential growth phase of the cell division cycle. Cells transformed by avian sarcoma virus (ASV) and other agents lost this activity regardless of the cell cycle phase. Cultures infected with transformation-defective ASV mutants retained a high level of fibrin clot retraction. However, this activity could not be recovered when temperature-sensitive, virus-transformed cells were shifted to a nonpermissive temperature (41 degrees C). The results indicated that, in this cell system, loss of fibrin clot retraction could be considered a reliable parameter of cell transformation.
正常禽胚成纤维细胞可诱导纤维蛋白凝块收缩。这种活性的高水平表达与细胞分裂周期的指数生长期相关。被禽肉瘤病毒（ASV）和其他因子转化的细胞无论处于细胞周期的哪个阶段都失去了这种活性。用转化缺陷型ASV突变体感染的培养物仍保留高水平的纤维蛋白凝块收缩活性。然而，当温度敏感的病毒转化细胞转移到非允许温度（41摄氏度）时，这种活性无法恢复。结果表明，在这个细胞系统中，纤维蛋白凝块收缩活性的丧失可被视为细胞转化的一个可靠参数。
