Krycève-Martinerie C, Lawrence D A, Crochet J, Jullien P, Vigier P
J Cell Physiol. 1982 Dec;113(3):365-72. doi: 10.1002/jcp.1041130304.
Chicken embryo fibroblasts and hamster BHK cells transformed by Rous sarcoma virus (RSV) release in their culture media growth factors which enhance markedly anchorage-independent colony formation in gelified medium, at the restrictive temperature (41 degrees 5 C), of chicken embryo fibroblasts (CEF) infected by RSV mutants with a ts mutation of the src gene. This action is not observed with uninfected CEF, and, therefore, appears to require some expression of the viral src gene in the target cells. The enhancing factors are proteins related to the family of the transforming growth factors (TGFs) by their molecular weight (about 20 kd), their heat and acid resistance, and their sensitivity to dithiothreitol. They do not compete with 125I EGF for binding on the EGF receptors of the membrane of A431 cells. As chicken embryo fibroblasts are devoid of EGF receptors, their activity is not potentiated by EGF.
经劳斯肉瘤病毒（RSV）转化的鸡胚成纤维细胞和仓鼠BHK细胞，在其培养基中释放生长因子，这些生长因子能显著增强src基因发生温度敏感（ts）突变的RSV突变体感染的鸡胚成纤维细胞（CEF）在41.5℃限制温度下于凝胶化培养基中形成非锚定依赖性集落的能力。未感染的CEF未观察到这种作用，因此，这似乎需要病毒src基因在靶细胞中有一定表达。这些增强因子是蛋白质，从其分子量（约20kd）、耐热性、耐酸性以及对二硫苏糖醇的敏感性来看，它们与转化生长因子（TGFs）家族相关。它们不与125I表皮生长因子（EGF）竞争结合A431细胞膜上的EGF受体。由于鸡胚成纤维细胞缺乏EGF受体，其活性不会因EGF而增强。
