一种通过在垂直转子中进行Percoll梯度离心从布氏锥虫中分离完整糖体的快速方法。

A rapid method for the isolation of intact glycosomes from Trypanosoma brucei by Percoll -gradient centrifugation in a vertical rotor.

作者信息

Opperdoes F R

出版信息

Mol Biochem Parasitol. 1981 Jul;3(3):181-6. doi: 10.1016/0166-6851(81)90048-7.

Abstract

A rapid method for the isolation of intact glycosomes from trypanosoma brucei is described. A post large-granule extract is subjected to density-gradient centrifugation on isotonic Percoll in a vertical rotor. This results in a good separation of the glycosomes, which equilibrated at 1.09 g/cm3, from other cellular components. Glycosomes isolated from such a gradient are 9-fold purified relative to the starting homogenate with a yield of 25%. Their glycolytic enzymes exhibit a high latency indicative of an intact glycosomal membrane.

摘要

本文描述了一种从布氏锥虫中快速分离完整糖体的方法。将大颗粒后提取物在垂直转子中于等渗Percoll上进行密度梯度离心。这使得糖体（在1.09 g/cm3处达到平衡）与其他细胞成分得到良好分离。从这样一个梯度中分离出的糖体相对于起始匀浆纯化了9倍，产率为25%。其糖酵解酶表现出高潜伏性，表明糖体膜完整。