Smith M
Nucleic Acids Symp Ser. 1980(7):387-95.
The applications of synthetic oligodeoxyribonucleotides to problems in molecular biology described in this article are those where the oligodeoxyribonucleotide is a probe for a specific region of a nucleic acid. This includes the isolation of the iso-1-cytochrome c gene of yeast; the sequence determination of RNAs and DNAs including regions of double-stranded DNA; the introduction of defined site-specific point mutations into bacteriophage OX174 and in the in vitro selection of mutant DNA from a mixture with wild-type DNA.
本文所述的合成寡脱氧核糖核苷酸在分子生物学问题中的应用，是指寡脱氧核糖核苷酸作为核酸特定区域的探针。这包括酵母同工-1-细胞色素c基因的分离；RNA和DNA（包括双链DNA区域）的序列测定；将特定的位点特异性点突变引入噬菌体OX174，以及从与野生型DNA的混合物中体外筛选突变DNA。
