Hadler H I, Demetriou M
Eur J Biochem. 1976 Oct 1;69(1):315-20. doi: 10.1111/j.1432-1033.1976.tb10888.x.
The addition of 2,2-dimethylsuccinic anhydride to mitochondrial suspensions fortified with gramicidin and potassium ion but without any permeant anion caused an immediate and rapid increase in volume (as indicated by absorbance change at 520 nm) and the uptake of potassium ion (as indicated by a cation-specific electrode). The phenomena was not inhibited by rutamycin but was inhibited by either rotenone, antimycin or 2,4-dinitrophenol. Rotenone inhibition was relieved by succinate thus one of the requirements of the process was energy derived from endogenous substrates. Potassium ion could be replaced by rubidium and cesium ions but not by lithium or sodium ions. Since 2,2-dimethylsuccinate could not replace the anhydride and was not a permeant anion there must also be a requirement for the anhydride bond. The action of the anhydride on the mitochondria must be direct. Only closely related anhydrides were capable of engendering the effect of a highly effective permeant anion.
向用短杆菌肽和钾离子强化但无任何渗透性阴离子的线粒体悬浮液中添加2,2-二甲基琥珀酸酐，会导致体积立即快速增加（通过520 nm处吸光度变化指示）以及钾离子摄取（通过阳离子特异性电极指示）。该现象不受鱼藤酮霉素抑制，但受鱼藤酮、抗霉素或2,4-二硝基苯酚抑制。鱼藤酮抑制作用可被琥珀酸缓解，因此该过程的一个要求是来自内源性底物的能量。钾离子可被铷离子和铯离子取代，但不能被锂离子或钠离子取代。由于2,2-二甲基琥珀酸不能替代酸酐且不是渗透性阴离子，所以对酸酐键也必有要求。酸酐对线粒体的作用必定是直接的。只有密切相关的酸酐能够产生高效渗透性阴离子的效果。
