Walker J R, Parish H A
Int Arch Allergy Appl Immunol. 1981;66(1):83-90. doi: 10.1159/000232803.
Lipoxygenase product formation by rabbit PMNs on exposure to the divalent ionophore A23187 was dependent upon pH, metabolic energy and substrate availability. Formation of 5-HETE and 5,12-diHETE (the major arachidonate oxygenation products) was inhibited by calcium depletion, by the metabolic inhibitors iodoacetate and 2-deoxyglucose, and by the substrate inhibitor TYA. The reaction had a pH optimum of 7.0-7.5. Colchicine, cytochalasin B and isoprenaline had no significant effects on HETE formation.
