Marcus A J, Broekman M J, Safier L B, Ullman H L, Islam N, Serhan C N, Weissmann G
Clin Physiol Biochem. 1984;2(2-3):78-83.
We studied interactions of human platelets and neutrophils with particular reference to the arachidonic acid pathway. Suspensions of [3H]-arachidonate-labeled platelets and unlabeled neutrophils were stimulated with ionophore A23187. We detected several radioactive arachidonate metabolites, which are not produced by platelets alone. This included [3H]-labeled leukotriene B4 (LTB4), dihydroxyeicosatetraenoic acid (DHETE) and 5-hydroxyeicosatetraenoic acid (5-HETE). When the platelet product, [3H]12-HETE, was added to ionophore-stimulated neutrophils, DHETE was formed. In addition, when [3H]5-HETE, a neutrophil arachidonate product, was added to stimulated platelets, DHETE was the major metabolite. We, therefore, suggest that upon stimulation, the platelet derived arachidonate can serve as precursor for the neutrophil-derived eicosanoids LTB4 and 5-HETE and that the platelet-derived product 12-HETE can be metabolized to DHETE by stimulated human neutrophils. It would appear that the platelet and neutrophil lipoxygenase pathways take part in cell-cell interactions - an observation which suggests a role for the neutrophils which are present in hemostatic plugs, thrombi and inflammatory processes.
我们研究了人类血小板与中性粒细胞的相互作用，特别关注花生四烯酸途径。用离子载体A23187刺激[3H] - 花生四烯酸盐标记的血小板和未标记的中性粒细胞悬浮液。我们检测到几种放射性花生四烯酸代谢物，这些代谢物不是血小板单独产生的。这包括[3H] - 标记的白三烯B4（LTB4）、二羟基二十碳四烯酸（DHETE）和5 - 羟基二十碳四烯酸（5 - HETE）。当将血小板产物[3H]12 - HETE添加到离子载体刺激的中性粒细胞中时，会形成DHETE。此外，当将中性粒细胞花生四烯酸产物[3H]5 - HETE添加到刺激的血小板中时，DHETE是主要代谢物。因此，我们认为在受到刺激时，血小板衍生的花生四烯酸可以作为中性粒细胞衍生的类花生酸LTB4和5 - HETE的前体，并且血小板衍生的产物12 - HETE可以被受刺激的人类中性粒细胞代谢为DHETE。似乎血小板和中性粒细胞的脂氧合酶途径参与了细胞间相互作用——这一观察结果表明存在于止血栓、血栓和炎症过程中的中性粒细胞具有一定作用。
