Feldberg R S, Carew J A
Int J Radiat Biol Relat Stud Phys Chem Med. 1981 Jul;40(1):11-7.
The radiation chemistry of nucleotide damage introduction into DNA gamma-irradiated in dilute aqueous solution has been studied with a damage-specific DNA binding protein. Irradiation in air, N2 or N2O in the presence and absence of free radical scavengers revealed that protein-recognizable lesions were introduced both by the hydrated electron and by the combination of the hydroxyl radical and superoxide anion radical, but not by the hydroxyl radical alone. In addition, nucleotide damage was introduced into DNA by an enzymatic superoxide-generating system.
利用一种损伤特异性DNA结合蛋白，研究了在稀水溶液中γ辐照的DNA引入核苷酸损伤的辐射化学。在有和没有自由基清除剂的情况下，在空气、氮气或一氧化二氮中进行辐照，结果表明，蛋白质可识别的损伤是由水合电子以及羟基自由基和超氧阴离子自由基的组合引入的，而不是仅由羟基自由基引入的。此外，通过酶促超氧生成系统将核苷酸损伤引入DNA。
