Sclafani R A, Wechsler J A
Mol Gen Genet. 1981;182(1):95-8. doi: 10.1007/BF00422773.
Isogenic dnaB, dnaG, and dnaB dnaG mutants were constructed and used as extracts in the cellophane-disc in vitro DNA replication system. The increased proportion of 5S DNA characteristics of the dnab extract and the lack of Okazaki piece synthesis characteristic of the dnaG extract were both apparent in analysis of the dnaB dnaG mutant extract reaction. A hypothetical scheme to explain these results and those of others is presented.
构建了同基因的dnaB、dnaG和dnaB dnaG突变体，并将其用作玻璃纸圆盘体外DNA复制系统中的提取物。在对dnaB dnaG突变体提取物反应的分析中，dnab提取物中5S DNA特征比例的增加以及dnaG提取物中冈崎片段合成缺乏的特征都很明显。本文提出了一个假设方案来解释这些结果以及其他结果。
