草履虫的纤毛膜囊泡含有电压敏感性钙通道。
Ciliary membrane vesicles of paramecium contain the voltage-sensitive calcium channel.
作者信息
Thiele J, Schultz J E
出版信息
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3688-91. doi: 10.1073/pnas.78.6.3688.
Abstract
Calcium influx into ciliary membrane vesicles from paramecia was measured by using arsenazo III as metallochromic indicator and as intravesicular calcium trap. Influx was rapid and reversible. In ciliary membrane vesicles from paramecium pawn mutants which lack the voltage-sensitive calcium channel, calcium influx was significantly less than in wild-type paramecia. These data demonstrate that the voltage-dependent calcium channel of paramecia is functional in a cell-free system and available for biochemical studies.
摘要
通过使用偶氮胂III作为金属显色指示剂和囊泡内钙捕获剂，测定了草履虫纤毛膜囊泡中的钙内流。钙内流迅速且可逆。在缺乏电压敏感性钙通道的草履虫pawn突变体的纤毛膜囊泡中，钙内流明显少于野生型草履虫。这些数据表明，草履虫的电压依赖性钙通道在无细胞系统中具有功能，可用于生化研究。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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