Krejcí P, Kleinwächter V, Koudelka J, Balcarová Z, Filkuka J, Löber G
Folia Biol (Praha). 1976;12(3):201-8.
Maximum binding capacity of calf thymus DNA and deoxyribonucleoprotein for methylene blue was investigated. The binding capacity of native and heat-denatured solutions of the biopolymers was compared with that of the solutions incubated with fixatives used in cytology, viz. 4% formaldehyde and methanol-acetic acid (3 : 1) mixture. The binding capacity of DNA and DNP is strongly reduced after the treatment with both fixation agents. Thermal denaturation leads to a small decrease in the binding capacity of DNA, but does not influence that of DNP. These results were compared with microphotometric determination of nuclear methylene blue content in thymocyte spreads. Fixation with MeAc increased the dye content as compared with air-dried thymocytes, whereas 4% formaldehyde exhibited practically no influence. The mechanisms connected with the different staining properties after incubating with fixation agents DNA and DNP solutions on the one hand and the nuclear material of thymocyte spreads on the other hand are discussed.
研究了小牛胸腺DNA和脱氧核糖核蛋白对亚甲蓝的最大结合能力。将生物聚合物的天然溶液和热变性溶液的结合能力与用细胞学中使用的固定剂（即4%甲醛和甲醇-乙酸（3:1）混合物）孵育的溶液的结合能力进行了比较。用两种固定剂处理后，DNA和DNP的结合能力均大幅降低。热变性导致DNA结合能力略有下降，但不影响DNP的结合能力。将这些结果与对胸腺细胞涂片细胞核亚甲蓝含量的显微光度测定结果进行了比较。与风干的胸腺细胞相比，用MeAc固定增加了染料含量，而4%甲醛实际上没有影响。讨论了一方面DNA和DNP溶液与固定剂孵育后不同染色特性，另一方面与胸腺细胞涂片核物质相关的机制。
