Aleshkin G I, Timakova N V, Ditiatkin S Ia, Il'iashenko B N, Skavronskaia A G
Genetika. 1981;17(7):1205-10.
Transformation of Escherichia coli K-12 for various chromosomal markers was accomplished by using AB1157 recBC+ strain as a recipient. The yield of transformants was reduced 10-fold, as compared with that obtained in JC7623 recBC sbcB recipient. Elimination of transformation has been obtained for arg, pro, his markers in AB1157 (pSA14) harbouring the R.M.EcoRI coding plasmid. Production of restriction endonuclease in this strain did not affect the efficiency of transformation for thr, leu markers. The presence of pSA25 which is isogenic to pSA14 but devoid of R.M.EcoRI genes has been irrelevant to transformation for leu, arg, pro, his, thr markers. Correlation between the restriction of transformed markers in vivo and in vitro is discussed.
以AB1157 recBC⁺菌株作为受体，实现了大肠杆菌K-12各种染色体标记的转化。与在JC7623 recBC sbcB受体中获得的转化子产量相比，转化子产量降低了10倍。在携带R.M.EcoRI编码质粒的AB1157（pSA14）中，已消除了arg、pro、his标记的转化。该菌株中限制性内切酶的产生不影响thr、leu标记的转化效率。与pSA14同基因但不含R.M.EcoRI基因的pSA25的存在与leu、arg、pro、his、thr标记的转化无关。讨论了体内和体外转化标记的限制之间的相关性。
