Skavronskaia A G, Aleshkin G I, Demkin V V
Genetika. 1979;15(10):1719-23.
The specifities of restriction of bacteriophages P1 and lambda controlled by R plasmids in Escherichia coli have been investigated. The isogenic strains harbouring the plasmids pAS26 coding for restriction endonuclease R.EcoRI, R245 coding for restriction endonuclease R.EcoRII and and R124 have been investigated in the present work. Modification-restriction controlled by R124 has been found to differ in specificity from those controlled by R245 and pAS26. Frequencies of restriction of bacteriophages P1vir and lambdavir specified by R124 pasmid differ from the frequencies in the strains harbouring pAS26 and R245 plasmids as well. The difference is due to the specifity of restriction-modification controlled by R124 plasmid. The data obtained are consistent with the determination of R124 specified restriction-modification activity as a novel one designated R.EcoRIII.
对大肠杆菌中由R质粒控制的噬菌体P1和λ的限制特异性进行了研究。在本研究中，对携带编码限制性内切酶R.EcoRI的质粒pAS26、编码限制性内切酶R.EcoRII的R245和R124的同基因菌株进行了研究。已发现由R124控制的修饰-限制在特异性上不同于由R245和pAS26控制的修饰-限制。由R124质粒指定的噬菌体P1vir和λvir的限制频率也不同于携带pAS26和R245质粒的菌株中的频率。这种差异是由于R124质粒控制的修饰-限制的特异性所致。所获得的数据与将R124指定的修饰-限制活性确定为一种新的命名为R.EcoRIII的活性一致。
