Nelson W J, Nelson S J, Traub P
Hoppe Seylers Z Physiol Chem. 1981 Jul;362(7):903-18. doi: 10.1515/bchm2.1981.362.2.903.
A simple method of analytical subcellular fractionation, combined with a sensitive computational method for data analysis and presentation, has been used to reinvestigate the distribution and relative amounts of several enzymes in the cytoplasmic and plasma membranes of two different cell types: one is a neoplastic, transformed cell type (Ehrlich ascites tumour cells), the other an untransformed, highly differentiated cell type (liver hepatocytes plus Kupffer and endothelial cells). In general the distribution of the enzymes in particular membranes is similar in the two cell types, however the relative amounts differ. Ehrlich ascites tumour cells have a higher specific activity of galactosyltransferase and ouabain-sensitive (Na,K)ATPase, while liver cells have higher glucose-6-phosphatase, 5'-nucleotidase and succinate dehydrogenase activity. These differences appear to be correlated with morphological and, in some cases, functional differences between the two cell types.
一种简单的亚细胞分级分离分析方法，结合一种用于数据分析和呈现的灵敏计算方法，已被用于重新研究两种不同细胞类型的细胞质膜和质膜中几种酶的分布和相对含量：一种是肿瘤性转化细胞类型（艾氏腹水瘤细胞），另一种是未转化的高度分化细胞类型（肝细胞加库普弗细胞和内皮细胞）。一般来说，两种细胞类型中特定膜内酶的分布相似，但相对含量不同。艾氏腹水瘤细胞具有较高的半乳糖基转移酶和哇巴因敏感的（钠，钾）ATP酶比活性，而肝细胞具有较高的葡萄糖-6-磷酸酶、5'-核苷酸酶和琥珀酸脱氢酶活性。这些差异似乎与两种细胞类型之间的形态学差异以及某些情况下的功能差异相关。
